Nitric oxide synthase in skeletal muscle fibres of patients with type 2 diabetes

Karla Punkt,Igor Buchwalow,Katharina Kandt,Andreas Oberbach,Volker Adams,Matthias Blueher

doi:10.1038/npre.2012.7049.1

Karla Punkt, Igor Buchwalow + Show 4 more

Open Access

https://doi.org/10.1038/npre.2012.7049.1

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Abstract

AbstractMuscle-derived nitric oxide (NO) mediates fundamental physiological actions on skeletal muscle including glucose uptake into muscle cells. Recently, we have shown that the altered glucose metabolism in skeletal muscle of patients with type 2 diabetes (T2D) is associated with changes in the metabolic profile of individual muscle fibres, but fibre-type specific changes in NO synthase (NOS) expression in skeletal muscle of T2D patients remain to be elucidated. Here we investigated fibre-type related NOS expression in vastus lateralis muscle of T2D patients compared with healthy individuals with normal glucose tolerance (NGT). Cytophotometrical assay and Western blotting did not reveal any quantitative differences between NOS expression in muscles from NGT and T2D subjects. Positive NOS immunoreactivity in vastus lateralis of T2D patients was found to be associated with fast-oxidative glycolytic (FOG) muscle phenotype. This indicates that NOS expression in T2D patients correlates both with skeletal muscle fibre type distribution and the activity of oxidative and glycolytic enzymes.

Highlights

Aim of the present study was to investigate the NOS1-3 expression in the vastus lateralis muscles of type 2 diabetes (T2D) patients in comparison to healthy individuals with normal glucose tolerance (NGT) and to show a relationship of NOS1-3 to defined fibre types
We investigated fibre-type related NO synthase (NOS) expression in vastus lateralis muscle of T2D patients compared with healthy individuals with normal glucose tolerance (NGT)
We have previously demonstrated that fast fibres without or very low oxidative metabolism fast glycolytic (FG) fibres were NOS-negative 8

Summary

Introduction

Aim of the present study was to investigate the NOS1-3 expression in the vastus lateralis muscles of T2D patients in comparison to healthy individuals with NGT and to show a relationship of NOS1-3 to defined fibre types. We investigated fibre-type related NOS expression in vastus lateralis muscle of T2D patients compared with healthy individuals with normal glucose tolerance (NGT). Positive NOS immunoreactivity in vastus lateralis of T2D patients was found to be associated with fast-oxidative glycolytic (FOG) muscle phenotype.

Results

Conclusion