Nitric oxide synthase- and neuropeptide Y-containing subpopulations of sympathetic neurons in the coeliac ganglion of the Atlantic cod, Gadus morhua, revealed by immunohistochemistry and retrograde tracing from the stomach

Abstract

In this study retrograde tracing was used to locate sympathetic ganglion cells innervating the stomach of a teleost fish, Gadus morhua. A subpopulation of small neurons in the coeliac ganglion was retrogradely labelled after Fast Blue injection in the stomach wall. Neurons projecting to the myenteric plexus and muscle layers contained tyrosine hydroxylase immunoreactivity, and neurons projecting to submucosal layers and blood vessels contained neuropeptide Y immunoreactivity in addition to being tyrosine hydroxylase immunoreactive. A population of nitric oxide synthase containing tyrosine hydroxylase immunoreactive neurons was also found in the coeliac ganglion. These neurons were not frequently labelled after injection in any layer of the stomach. The presence of entero–enteric pathways was also surveyed, but too few enteric neurons were labelled with Fast Blue after injection in the coeliac ganglion to indicate a presence of an entero–enteric reflex. We conclude that in teleost fish, as previously reported in a variety of mammals, a pattern of target specific chemical coding of sympathetic neurons exists, but that all reflex systems of mammalian vertebrates are perhaps not present in fish.