Abstract
The effects of nitrix oxide (NO) released from zwitterionic polyamine/NO adducts on Cu 2+-induced low density lipoprotein (LDL) oxidation were studied. When each of the two kinds of NO releasing zwitterionic polyamine/NO adducts (NOC5 and NOC7) was incubated at 5 μM with isolated human LDL (0.25 mg/ml) and Cu 2+, the formation of thiobarbituric acid reactive substances (TBARS) was inhibited. The duration of inhibition by NOC7 (20 min) and NOC5 (100 min) corresponded to the NO generation lives of respective zwitterionic polyamine/NO adducts. The duration of inhibition was dependent on the amount of NOC5 added (2.5–20 μM). Repeated additions of 5 μM NOC5 at 100 min intervals worked as inhibitor in the same manner. NOC5 broke to inhibit at any process of the Cu 2+-induced LDL oxidation reaction. Fragmentation of apolipoprotein B derived from Cu 2+-induced LDL oxidation was also prevented by the addition of NOC5. These results clearly indicate that NO inhibits the oxidative modification of LDL induced by Cu 2+. NO releasing zwitterionic polyamine/NO adducts are good reagents for NO studies.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.