Nitric Oxide Regulates Mitochondrial Fatty Acids and Promotes CBF Expression of Peach Fruit during Cold Storage

Xiaoshan Guo,Jianrong Feng,Dandan Huang,Shuhua Zhu,Siyu Wen,Yang Bai

doi:10.32604/phyton.2022.018022

Xiaoshan Guo, Jianrong Feng + Show 4 more

Open Access

https://doi.org/10.32604/phyton.2022.018022

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Journal: Phyton	Publication Date: Jan 1, 2022
Citations: 4	License type: cc-by

Affiliation: Shihezi University, Shandong Agricultural University

Abstract

Low-temperature storage is convenient for postharvest preservation of peach fruit, but peach fruit is sensitive to cold damage, which lowers its quality. Nitric oxide (NO) has the potential to improve the bitter resistance of peach fruit. In this work, peach fruit was treated with 15 μmol L−1 NO and 5 μmol L−1 c-PTIO [2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxo-3-oxide], to study changes in mitochondrial fatty acids and expression of the C-repeat binding factor (CBF). The results showed that 15 μmol L−1 exogenous NO significantly maintained fruit quality, reduced peroxidation of mitochondrial fatty acids, increased the activities of the antioxidants superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbic acid peroxidase (APX), and reduced the content of hydrogen peroxide (H2O2). Meanwhile, NO treatment suppressed the increase in browning index and ion leakage rate, increased the activity of S-nitrosoglutathione reductase (GSNOR), the contents of S-nitrosothiols (SNOs), and the ratios of mitochondrial NAD+/NADH and NADP+/NADPH, increased the expression levels of PpCBF1/5/6. However, the expression levels of PpCBF2/3/4 were not significantly regulated by exogenous NO. Peaches treated with c-PTIO showed opposite effects to those treated with exogenous NO. These results suggest that exogenous NO can improve antioxidant capacity, preserve mitochondrial fatty acids, and upregulate the expression of PpCBF1/5/6 to alleviate cold tolerance and maintain the peach quality during storage.

Highlights

Peach fruits (Prunus persica) are delicious, nutritious and loved by consumers around the world, but they deteriorate quickly at room temperature
Based on the fact that Nitric oxide (NO) is a major regulator in plants in reaction to cold stress, and is specific in response to cold stress, we used the endogenous NO scavenger c-PTIO to verify the role of endogenous NO treatment on the physiological regulatory mechanisms of frozen peach fruit
We demonstrated that 15 μmol L−1 of NO remarkable reduced the browning index and ion leakage rate during storage of peaches, increased GSNOR activity leading to the accumulation of SNOs, increased the NAD+/NADH and NADP+/NADPH ratios, maintained a high mitochondrial unsaturated fatty acid index and enhanced the expression of PpCBF1/5/6

Summary

Introduction

Peach fruits (Prunus persica) are delicious, nutritious and loved by consumers around the world, but they deteriorate quickly at room temperature. Peach fruits are exposed to low temperatures during continuous cold storage, and are prone to cold damage [1]. Cold damage is the most important factor affecting the quality and lifespan of peach fruits during cold storage, characterized by loss of taste and maturing capacity, rotting, pulp breakage, lack of fleshy quality, and pulp discoloration [2,3]. Both membrane fatty acids and C-repeat binding factors (CBFs) contribute to the reaction of plants to cold stress.

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