Nitric oxide metabolites in patients with asthma: induced sputum versus blood

Abstract

Nitric oxide (NO) plays an important role in physiological regulation of the airways. The monitoring of airway inflammation has being observed in bronchial asthma directly, by sputum examination, and indirectly, by measurements in peripheral blood. To investigate the diagnostic value of these two methods, we compared NO metabolites in induced sputum and serum obtained in patients with asthma and control subjects. Hypertonic saline induced sputum and serum were obtained in 13 patients with asthma and 10 control subjects. NO metabolite level was assayed by using modified Griess reaction. Eosinophil cationic protein (ECP) was measured by fluoroimmunoassay, and detected interleukin (IL)-5 by a sandwich ELISA. The accuracy of the tests was measured by plotting the data in receiver operating characteristic (ROC) curves and comparing the area under the curve for NO metabolites. Asthmatic patients, compared with control subjects, had significantly higher NO metabolites in induced sputum (1252·5±203·3 moll −1 vs. 557·2±101±.5 mol l −1, P < 0·01) but not in serum. IL-5 in induced sputum was detected more frequently in patients with asthma than in control subjects [ 11 13 (84·6%) vs. 1 10 (10%), P < 0·01]. Asthmatic patients, compared with control subjects, had significantly higher ECP concentration in induced sputum (1270·0 ± 197·9 g 1 vs. 154·6 ± 47·4 g l −1, P < 0·01). There were significant positive correlations between NO metabolites in induced sputum and eosinophils, ECP in induced sputum ( r=0·58 P<0·05; r=0·64, P<0·01) in patients with asthma but not in serum. The area under the ROC curve showed that NO metabolites in induced sputum (0·78) are more accurate marker than NO metabolites in serum (0·53) ( P<0·05). These findings suggest that NO metabolites in induced sputum is a more valuable indicator to monitor asthmatic airway inflammation than those in serum.