Nitric oxide and asymmetric dimethyl arginine (ADMA) levels in an experimental hydronephrotic kidney caused by unilateral partial ureteral obstruction.

Cabir Alan,Naci Topaloğlu,Ahmet Reşit Ersay,Hasan Anil Kurt,Dilek Ülker Çakir,Gökhan Baştürk

doi:10.1590/s1677-5538.ibju.2015.0030

Abstract

ABSTRACTAim Our aim is to measure asymmetric dimethyl arginine and nitric oxide levels in rats with induced unilateral acute ureteral obstruction to research the effects on the kidney.Material and Methods The study included 21 adolescent (average age 6 weeks) Sprague-Dawley male rats weighing between 240-290g divided at random into 3 groups. Group-1: Control group (n=6): underwent no procedures. Group-2: Sham group (n=6): underwent the same procedures as the experimental group without ureter and psoas muscle dissection. Group-3: Group with induced partial unilateral ureteral obstruction (n=9). All rats were sacrificed after 12 weeks. Superoxide dismutase enzyme activity and nitrite and nitrate salt levels were measured in renal tissue. Plasma nitrite-nitrate and ADMA levels were examined.Results In the experimental group histopathological changes observed included renal pelvis dilatation, flattened papillae, sclerotic glomerulus and fibrosis. In the experimental group tissue SOD and blood ADMA levels were higher than the control and sham groups (p<0.05) while tissue NO and plasma NO values were lower than in the sham and control groups (p<0.05).Conclusion Oxidative stress and disruption of NO synthesis play an important role in renal function and histopathological changes after obstructive renal disease. To prevent renal complications developing after obstructive nephropathy we believe that a new strategy may be research on reducing ADMA.

Highlights

Ureteral obstructions are frequently observed in urology practice and without early diagnosis and treatment it may cause some serious complications [1,2,3,4]
While there was no pathological changes in the kidney sections from the control group and sham group, in the experimental group induced partial unilateral ureteral obstruction (PUUO), on histopathological examination of the H&E dyed kidney fibrosis, inflammation, flattening of the renal papillae and dilatation of the renal pelvis were observed with varying degrees On kidney sections stained with periodic acid-Schiff (PAS) sclerotic glomerular changes, inflammation and fibrotic changes were observed (Figure-1)
When compared the experimental group to control and sham groups, we found increased both Asymmetric dimethyl arginine (ADMA) and superoxide dismutase (SOD) levels (p

Summary

Introduction

Ureteral obstructions are frequently observed in urology practice and without early diagnosis and treatment it may cause some serious complications [1,2,3,4]. Many experimental studies have shown that retrograde glomerular reflux developing due to ureteral obstruction disrupts NO synthesis and in parallel affects renal function How and by which mechanism NO synthesis is disrupted is still not fully known. Asymmetric dimethyl arginine (ADMA) is a nitric oxide synthesis (NOS) inhibitor [9]. The function of NOS in the body is provided by NO synthesis from L-arginine. In this reaction, occurring in the vascular endothelium, ADMA inhibits NOS activity by preventing L-arginine uptake into cells. In other words ADMA regulates the rate of ibju | Asymmetric Dimethyl Arginine and Hydronephrosis

Objectives

Methods

Results

Conclusion