Nitratireductor lacus sp. nov., isolated from Yuncheng Salt Lake, China.

Abstract

This is the taxonomic study of a novel bacterial strain, designated GSS14T, isolated from a sediment sample of Yuncheng Salt Lake, China. Cells were Gram-negative, ovoid to rod-shaped and motile by means of flagella. The isolate could grow at 10-45 °C, at pH 6.5-11.0 and in the presence of 0-12 % (w/v) NaCl. The dominant fatty acids were summed feature 8 (consisting of C18 : 1ω7c and/or C18 : 1ω6c; 76.7 %) and the DNA G+C content was 61 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GSS14T was affiliated with the genus Nitratireductor, and it was most closely related to Nitratireductor kimnyeongensis KY 101T (98.2 % 16S rRNA gene sequence similarity) and Nitratireductor aquibiodomus JCM 21793T (96.6 %). DNA-DNA hybridization between strains GSS14T and N. kimnyeongensis KY 101T revealed 52 % relatedness. Phenotypic, chemotaxonomic and phylogenetic data support assignment of this isolate to the genus Nitratireductor as a representative of a novel species. The name Nitratireductor lacus sp. nov. is proposed, with strain GSS14T (=KCTC 52186T=MCCC 1K02481) as the type strain.