Abstract
Methods and results BALB/c mice were fed untreated (OVA), sham-nitrated (snOVA) or nitrated ovalbumin (nOVA) with or without concomitant acid-suppression. To analyze systemic effects, mice were injected the allergens intraperitoneally (i.p.). Animals being fed OVA or snOVA with antiacids developed elevated IgE, IgG1 and IgG2a titers. Oral immunizations of nOVA under acid-suppression did not result in IgG and IgE formation. However, all i.p. immunized mice revealed high levels of IgE, which were significantly increased in the group being injected nOVA. In RBL-assays, all groups with OVA-specific IgE showed a significant increased mediator release with nOVA as trigger compared to OVA. To analyze the immune response in the involved organ, gastric tissues were screened for cytokine expression by real-time-PCR. Only the acid-suppressed groups being fed OVA or snOVA revealed a higher expression of Th2 and inflammatory markers. In gastric digestion experiments, nOVA was degraded within minutes, whereas OVA and snOVA remained stable up to 120min. Additionally, HPLCchip-MS/MS analysis revealed the most efficiently nitrated tyrosine residue within an ovalbumin epitope recognized exclusively after oral immunization.
Highlights
Nitration e.g. by ambient pollutants was demonstrated to increase the allergenicity of the major birch pollen allergen Bet v1
Oral immunizations of nitrated ovalbumin (nOVA) under acid-suppression did not result in IgG and IgE formation
All i.p. immunized mice revealed high levels of IgE, which were significantly increased in the group being injected nOVA
Summary
Susanne C Diesner1*, Eva Untersmayr, Gertie J Oostingh, Kathrin Selzle, Tobias Pfaller, Cornelia Schultz, Yingyi Zhang, Durga Krishnamurthy, Philipp Starkl, Regina Knittelfelder, Elisabeth Förster-Waldl, Arnold Pollak, Otto Scheiner, Ulrich Pöschl, Erika Jensen-Jarolim, Albert Duschl. From Food Allergy and Anaphylaxis Meeting 2011 Venice, Italy. From Food Allergy and Anaphylaxis Meeting 2011 Venice, Italy. 17-19 February 2011
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