Nitrated hydrochar reduce the Cd accumulation in rice and shift the microbial community in Cd contaminated soil

Abstract

Rice grown on Cd-contaminated soil may accumulate Cd in grain, which is extremely harmful to human health. Several managements are developed to reduce the Cd load in rice, while in-situ immobilization by soil amendments has been attractive for its feasibility. Waste-derived hydrochar (HC) has been shown effective at immobilizing Cd in soil. However, potential plant negative effects and huge application amount are crucial to resolving in extensive application of HC. Nitric acid ageing may be an effective method to deal with these problems. In this paper, HC and nitrated hydrochar (NHC) were added to the Cd-contaminated soil at rates of 1% and 2% in a rice-soil column experiment. Results showed that NHC markedly promoted root biomass of rice by 58.70–72.78%, whereas HC had effects of 35.86–47.57%. Notably, NHC at 1% reduced the accumulation of Cd in rice grain, root and straw by 28.04%, 15.08% and 11.07%, respectively. A consistent decrease of 36.30% in soil EXC-Cd concentration was caused by NHC-1%. Following soil microbial community was shifted greatly under HC and NHC applications. The relative abundance of Acidobacteria was decreased by 62.57% in NHC-2% and by 56.89% in HC-1%. Nevertheless, Proteobacteria and Firmicutes were promoted by NHC addition. In contrast to HC, co-occurrence network of dominated bacteria was more complex and centralized generated by NHC. Key bacteria in that metabolic network of NHC such as Anaerolineae and Archangiaceae played key roles in Cd immobilization. These observations verified that NHC was more efficient to decrease Cd accumulation in rice and could alleviate the negative roles to plant by microbial changings in community composition and network. It could provide an enrichment of paddy soil microbial responds to the interaction of NHC with Cd and lay a foundation for the remediation of Cd-contaminated soil by NHC.