Abstract
The uptake of nitrate by seedlings of common buckwheat ( Fagopyrum esculentum) increased gradually with time ( t) and with increasing medium nitrate concentration ( c) up to t 60 minutes and c 1mM, respectively. Glucose had a stimulatory effect on nitrate uptake. 3(3,4-Dichlorophenyl 1,1-dimethylurea) (DCMU) markedly suppressed the uptake of nitrate by buckwheat seedlings. NH 4 + and ClO 3 − ions also suppressed nitrate uptake. While the inhibition due to NH 4 + was non-competitive in nature, the inhibition due to ClO 3 − ions was competitive. Uptake of nitrate was also markedly affected by pH of the nutrient medium, with the optimum uptake at near neutral pH.
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