Abstract
Selected strains of Rhizobium japonicum have been reported to produce hydrogenase [1] and more recently, grow chemolithotrophically in a mineral salts medium, utilizing hydrogen and carbon dioxide as respective energy and carbon sources [2]. The synthesis of uptake hydrogenase by R. japonicum is sensitive to the presence of oxygen. During chemolithotrophic growth, the concentration of oxygen must be low or the formation of the hydrogen uptake system is rapidly repressed [2,3]. Nitrate has been shown to serve as the electron acceptor during chemoorganotrophic growth by cowpea rhizobia and R. japonicum [4,5]. We have been able to grow R. japonicum under anaerobic conditions chemolithotrophically with nitrate serving as the electron acceptor [6]. Hydrogen and carbon dioxide were utilized as energy and carbon sources respectively, and dissimilation of nitrate occurred. Here, we report the effect of nitrate, nitrite, and nitrous oxide, as compared to oxygen, upon hydrogen uptake by R. japonicum grown chemolithotrophically under anaerobic conditions. 2.1. Bacterial strains
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