Nitrate assimilatory properties of barley grown under long‐term N limitation: Effects of local nitrate supply in split‐root cultures

Abstract

The effect of nitrate availability on characteristics of the nitrate assimilatory system was investigated in N‐limited barley (Hordeum valgare L. cv. Golf), grown with the seminal root system split into initially equal‐sized halves. The cultures were continuously supplied with nitrate‐N at a relative addition rate (RA) of 0.09 day−1, which resulted in relative growth rates (RG) that were ca 85% of those observed under surplus nitrate nutrition. The total N addition was divided between the subroots in ratios of 100:0, 80:20, 70:30, 60:40, and 50:50. For comparison, standard cultures were grown at RAs ranging from 0.03 to 0.18 day−1. Initially, biomass and N partitioning to the subroots responded strongly and proportionally to the nitrate distribution ratio. After 12‐14 days no further effect was observed. The Vmax for net nitrate uptake and in vitro nitrate reductase (NR) activity were measured in acclimated plants, i.e., after > 14 days under a certain nitrate regime. In subroots fed from 20 to 100% of the total N addition, Vmax for net nitrate uptake increased slightly, whereas NR activity was unaffected. Uptake and NR activities were insignificant in the 0%‐subroot. Uneven nitrate supply to individual subroots had negligible effect on the whole‐plant ability for nitrate uptake, and the relative Vmax (unit N taken up per unit N in whole plant tissue and time) remained about 7‐fold in excess of the demand set by growth. Balancing nitrate concentrations (the resulting external nitrate concentrations at a certain RA) generally ranged between 2 and 10 μM at growth‐limiting RA, both when predicted from uptake kinetics and when actually measured. When comparing split root and standard cultures when acclimated, it appears that uptake and NR activities in roots respond more strongly to over‐all nitrate availability than to nitrate availability to individual subroots.