Abstract
In this study, nisin production has been enhanced by using batch and fed-batchfermentation with calcium-alginate immobilized cell culture. Due to the detrimental effects of originalphosphate rich growth medium on the immobilizing beads, an altered complex growth medium fornisin production was used. Various pH profiles were evaluated for both batch and fed-batchfermentations. For batch fermentations, a 2.1 fold higher nisin activity was obtained by allowing thepH to drop freely after 4 hrs of fermentation at constant pH. A step-wise pH profile exhibited adetrimental effect on nisin production during batch fermentations. For fed-batch fermentations, a 2.9fold higher nisin activity was obtained by allowing the pH to remain at a constant pH of 6.8. Theresults clearly showed that immobilized cell culture can be used in order to improve nisinfermentation for both batch and fed-batch fermentation.
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