NIR-I fluorescence imaging tumorous methylglyoxal by an activatable nanoprobe based on peptide nanotubes by FRET process

Abstract

Methylglyoxal (MGO), a glycolysis metabolite with high reactivity, can nonenzymatically modify proteins, lipids and nucleic acids etc., and it is closely related to the development of tumors. The accurate detection and high-performance optical imaging of MGO from deep tumor issues is of great significance for understanding their roles in tumor initiation and progression. Herein, we have presented a nanoprobe D/I-PNTs with emission in the first near infrared (NIR-I) region by employing a fluorescence resonance energy transfer (FRET) process between a far-red emission MGO probe and IR783 based on peptide nanotubes. The nanoplatform extended the emission range of MGO probe through FRET process and avoided complex molecular design and synthesis. The biocompatible peptide nanotubes improved the water solubility of MGO probe. D/I-PNTs was sensitive to MGO with a detection limit of 272 nM and enabled high-resolution NIR-I fluorescence imaging of MGO induced by glyoxalase I (GLO1) inhibitor in tumor with higher penetration depth (∼4 mm) than that in visible (Vis) region (∼3 mm). Most importantly, the FRET process based on the structure characteristics of peptide nanotubes can be a universal approach to realize the extension of emission wavelength and ratio detection of target analytes, which will be a promising strategy for bioimaging in deep tissue with high contrast.