NIR Photosensitizer for Two-Photon Fluorescent Imaging and Photodynamic Therapy of Tumor.

Lujia Chen,Meijuan Chen,Changsheng Ye,Ruiyuan Liu,Yuping Zhou

doi:10.3389/fchem.2021.629062

Lujia Chen, Meijuan Chen + Show 3 more

Open Access

https://doi.org/10.3389/fchem.2021.629062

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Abstract

Preparation of near-infrared (NIR) emissive fluorophore for imaging-guided PDT (photodynamic therapy) has attracted enormous attention. Hence, NIR photosensitizers of two-photon (TP) fluorescent imaging and photodynamic therapy are highly desirable. In this contribution, a novel D-π-A structured NIR photosensitizer (TTRE) is synthesized. TTRE demonstrates near-infrared (NIR) emission, good biocompatibility, and superior photostability, which can act as TP fluorescent agent for clear visualization of cells and vascular in tissue with deep-tissue penetration. The PDT efficacy of TTRE as photosensitizer is exploited in vitro and in vivo. All these results confirm that TTRE would serve as potential platform for TP fluorescence imaging and imaging-guided photodynamic therapy.

Highlights

Photodynamic therapy (PDT) as a noninvasive treatment procedure has attracted enormous attention due to its selective destroy of local lesions (Dai et al, 2019; Li et al, 2016; Kwiatkowski et al, 2018)
We develop a D-π-A structured NIR photosensitizer (TTRE), which was rationally designed as electron-donating triphenylamine as electron-donating group, rhodanic as electron-withdrawing units, and thiophenyl as π bridge
TTRE exhibited NIR emission, reactive oxygen species (ROS) generation ability, and two-photon fluorescent imaging capacity. Both in vitro and in vivo studies confirmed that TTRE has effective anticancer potential and is amenable to imagingguided photodynamic therapy of tumor

Summary

INTRODUCTION

Photodynamic therapy (PDT) as a noninvasive treatment procedure has attracted enormous attention due to its selective destroy of local lesions (Dai et al, 2019; Li et al, 2016; Kwiatkowski et al, 2018). Various NIR photosensitizers have been prepared for imaging-guided PDT of tumor, including porphyrin, chlorin, phthalocyanine, and BODIPY derivatives (Liu et al, 2016; Pan et al, 2019; Szurko et al, 2020; Zheng et al, 2020a) These NIR photosensitizers suffer from several intrinsic drawbacks, such as small Stokes’ shift, poor photostability, and unsatisfied biocompatibility. Both in vitro and in vivo studies confirmed that TTRE has effective anticancer potential and is amenable to imagingguided photodynamic therapy of tumor

MATERIAL AND METHODS

AND DISCUSSION

Findings

CONCLUSION