Abstract
Nipah virus causes periodic livestock and human disease with high case fatality rate, and consequent major economic, social and psychological impacts. Fruit bats of the genus Pteropus are the natural reservoir. In this study, we used real time PCR to screen the saliva and urine of P. vampyrus from North Sumatera for Nipah virus genome. A conventional reverse transcriptase (RT-PCR) assay was used on provisionally positive samples to corroborate findings. This is the first report of Nipah virus detection in P. vampyrus in Sumatera, Indonesia.
Highlights
Nipah virus (NiV) is a novel paramyxovirus that is pathogenic to pigs and humans
In this paper we report the detection of Nipah virus genome in P. vampyrus in Sumatera, Indonesia using real time PCR
We targeted saliva and urine samples from Pteropus vampyrus in northern Sumatera based on the findings of Johara et al, 2001 [1] and Chua et al, 2002 [18], and adapting the methods of Wacharapluesadee et al, 2005, 2006 [11,20]
Summary
Nipah virus (NiV) is a novel paramyxovirus that is pathogenic to pigs and humans. The first attributed outbreak was reported in1998–9 in Malaysia, causing respiratory disease in pigs. Nipah virus (NiV) is a novel paramyxovirus that is pathogenic to pigs and humans. 2001 [1] suggested (based on their serologic findings) that flying-foxes were the likely natural reservoir of Nipah virus in Malaysia, and beyond. This contention was strengthened following the detection of Nipah virus genome in the urine and saliva of P. hypomelanus and P. vampyrus in Malaysia [2,4]. Nipah infection has subsequently been reported in Pteropus spp. across their global distribution, strongly suggesting that Nipah and related viruses have a long association with bats of this genus [5]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
