Niños nacidos de técnicas de reproducción asistida con espermatozoides no procedentes de eyaculado

Abstract

IntroductionThe objective of this study is to report our experience in ARTs with non-ejaculated sperm and follow-up of the children born. These results show the need to update the appendix I of the Law 14/2006, 26th of May, which includes these types of techniques. MethodsRetrospective study on 80 cycles for 57 couples. Male pathology can be classified into: obstructive azoospermia (OA) (35); non-obstructive azoospermia (NOA) (13); Anejaculation (9). Sperm obtained by epididymal aspiration or testicular biopsy were cryopreserved before the FIVICSI cycle. After thawing, it was enzyme treated (in cases of testicular sperm), and subsequent activation of motility with pentoxifylline “in situ”. Fertilisation, implantation, pregnancy progress, abortion, single and multiple gestations were evaluated. For information about the obstetric and evolutionary development of children born a telephone survey was carried out on couples who were pregnant. ResultsFrom the 80 cycles, and 947 oocytes obtained, the fertilisation rate was 60.2%. From these, 196 embryos were transferred and 130 embryos were cryopreserved. The overall pregnancy rate was 46.2% and 63.7% per cycle per patient. The implantation rate was 26%. In freeze-thaw cycles, 44 embryos were transferred in 23 cycles; the pregnancy rate was 22.7% per cycle and 33.3% per patient. The overall abortion rate was 19.1%. In general, couples replied that their children were healthy and were developing well. ConclusionsThe introduction of using non-ejaculated sperm in the laboratory is a routine technique, with good results and the birth of healthy children, as long as previous studies are carried out on each case.