NiO-nanoparticles induce reduced phytotoxic hazards in wheat (Triticum aestivum L.) grown under future climate CO2

Abstract

Due to industrialization and expansion of nanotechnology, ecosystem contamination by nanoparticles is likely. Overall, nanoparticles accumulate in environmental matrices and induce phytotoxicity, however future climate (elevated CO2 (eCO2)) may affect the distribution of nanoparticles in ecosystems and alter their impact on plants. In the current study, nickel oxide nanoparticles (NiO-NPs) with an average diameter of 54 nm were synthesized by chemical pericipitation method using Triton X-100 and characterized by scanning electron microscopy (SEM), UV-VIS spectroscopy and Fourier transform infrared spectroscopy (FTIR). We have investigated the impact of NiO-NPs at a concentration of 120 mg kg−1 soil, selected based on the results of a preliminary experiment, on accumulation of Ni ions in wheat (Triticum aestivum L.) and how that could influence plant growth, photosynthesis and redox homeostasis under two CO2 scenarios, ambient (aCO2, 400 ppm) and eCO2 (620 ppm). NiO-NPs alone reduced whole plant growth, inhibited photosynthesis and increased the levels of antioxidants. However, improved defense system was not enough to lessen photorespiration induced H2O2 accumulation and oxidative damage (lipid and protein oxidation). Interestingly, eCO2 significantly mitigated the phytotoxicity of NiO-NPs. Although, eCO2 did not affect Ni accumulation and translocation in wheat, it promoted photosynthesis and inhibited photorespiration, resulting in reduced ROS production. Moreover, it further improved the antioxidant defense system and maintained ASC/DHA and GSH/GSSG redox balances. Organ specific responses to NiO-NPs and/or eCO2 were indicated and confirmed by cluster analysis. Overall, we suggest that wheat plants will be more tolerant to NiO-NPs stress under future climate CO2.