Abstract
To systematically study the chemical constituents in Magnolia officinalis var. biloba fruits, nine phenylethanoid glycosides were isolated by solvent extraction, silica gel, and preparative high-performance liquid chromatography (HPLC). Their structures were elucidated by 1D and 2D NMR analyses, including COSY, HMQC and HMBC correlations, and HPLC analysis of sugar residue. Nine phenylethanoid glycosides, namely, magnoloside Ia (1), magnoloside Ic (2), crassifolioside (3), magnoloside Ib (4), magnoloside IIIa (5), magnoloside IVa (6), magnoloside IIa (7), magnoloside IIb (8) and magnoloside Va (9), were first isolated from the n-butanol fraction of Magnolia officinalis var. biloba fruits alcohol extract. Free radical scavenging activities of the nine phenylethanoid glycosides were assessed using the DPPH, ABTS, and superoxide anion radical scavenging assays. Simultaneously, protective effects of all compounds against free radical-induced oxidative damage were evaluated by two different kinds of mitochondrial damage model. The protective effects were assessed by mitochondrial swelling, the formations of malondialdehyde (MDA) and lipid hydroperoxide (LOOH), the activities of catalase (CAT), glutathione reductase (GR) and superoxide dismutase (SOD). All phenylethanoid glycosides showed significant protective effects.
Highlights
Magnolia bark extracts are derived from Magnolia officinalis Rehd. et Wils. (M. officinalis) or Magnolia officinalis Rehd. et Wils. var. biloba Rehd. et Wils. (M. officinalis var. biloba)
Whether the phenylethanoid glycosides isolated from M. officinalis var. biloba fruits have the same effects as those isolated from Orobanchaceae plants needs to be elucidated
In view of the more abundant resources of M. officinalis var. biloba, we focused on the n-butanol fraction of the alcohol extract from M. officinalis var. biloba fruits and isolated nine phenylethanoid glycosides (Fig. 1)
Summary
Magnolia bark extracts are derived from Magnolia officinalis Rehd. et Wils. (M. officinalis) or Magnolia officinalis Rehd. et Wils. var. biloba Rehd. et Wils. (M. officinalis var. biloba). The isolation and structural elucidation of these nine phenylethanoid glycosides, as well as their free radical scavenging activities, are reported in this paper.
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