Abstract
Adsorptive Stripping Voltammetric method for Nimesulide (NIM) determination was developed. As a working electrode, glassy carbon electrode (GCE) modified with carbon black and Nafion (CB-Nafion GCE) was used. All measurements were carried out in 0.1 M acetate buffer (pH 4.6). Conducted experiments allowed to optimize differential pulse voltammetry (DPV) instrumental parameters: sampling and waiting time ts = tw = 10 ms, step potential Es = 4 mV, and pulse amplitude ΔE = 50 mV. The best results were obtained for preconcentration potential and time equal to 400 mV and 20 s, respectively. Limit of detection was calculated and was equal to 0.14 µM for 20-s preconcentration time and 0.06 µM for 40-s preconcentration time. In order to prove the applicability of the developed method, concentration of nimesulide in pharmaceutical products was determined. Calculated recoveries were in the range 94–99%, which indicates that the method might be assumed as accurate. Coefficient of variation was equal to 5.0% (n = 7, NIM concentration 1 µM) Obtained results of NIM determination were in good agreement with the content declared by producers.
Highlights
Non-steroidal anti-inflammatory drugs (NSAIDs) are a group of substances which are inhibitors of cyclooxygenase (COX)
Typical three-electrode system was used: a working electrode, reference electrode (Ag/AgCl/3 M KCl), and auxiliary electrode
Peak current and potential measured on the surface of each modified electrode were as follow: glassy carbon electrode (GCE) modified with carbon black and Nafion 1032 mV and 3.95 μA, GCE modified with carbon black: 1038 mV and 0.83 μA, and GCE modified with graphene and platinum 1060 mV and 0.31 μA
Summary
Non-steroidal anti-inflammatory drugs (NSAIDs) are a group of substances which are inhibitors of cyclooxygenase (COX). One of the non-steroidal anti-inflammatory drugs commonly prescribed for painful conditions is nimesulide It belongs to the group of sulfonanilides [1,2,3]. The most common are high-performance liquid chromatography (HPLC) [4, 5], liquid chromatography with UV detection (LC-UV) [6], liquid chromatography coupled with mass spectrometry (LC–MS) [7, 8], and spectrophotometry [9,10,11] Those methods are often complicated, require expensive equipment, and generate toxic chemical wastes. Glassy carbon electrode (GCE) and carbon paste electrodes (CPE) are the group of WE Their main advantage is the possibility of their modification with a wide group of materials.
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