Nil tilapiası (Oreochromis niloticus) lenfosit sitosolik protein 1 (lcp1) geninin biyoinformatiği

Abstract

Bioinformatics analysis of lymphocyte cytosolic protein 1 (lcp1) gene in tilapia (Oreochromis niloticus) which is a model organism in experimental studies were completed in this study. For this purpose, characterization and identification of lcp1 has been completed and ensembl database has been used to design the structure of lcp1 gene. In addition, the chromosome region of tilapia lcp1 and other genes in the same region with lcp1were determined. The chromosome of these genes were detected in zebrafish and human which are identical orthologos of tilapia. Conserved gene synteny designed manually according to these chromosomal regions. In addition, amino acid sequences synthesized by lcp1 gene in some vertebrates were determined using some bioinformatics databases such as UNIPROT, ENSEMBL and NCBI before determine the phylogenetic relationship between these organisms and tilapia. Sequence similarity-identity rate of tilapia lcp1 gene with zebrafish, rainbow trout, human, mouse and platyfish lcp1/Lcp1 was calculated using BLOSUM62 matrix algorithm. This study is of great importance for the completion of in silico analysis of lcp1 gene in tilapia because it is an aquatic model organism and it has an important place among economic aquaculture species. However this study provides the basic pioneering information for the future studies on molecular stress response in fish.