Abstract
The NifH protein is a subunit of the nitrogenase enzyme that catalyzes the reduction of atmospheric nitrogen to ammonia. This protein contains highly conserved regions including the nucleotide binding sites, metal center ligands and the Switch I and Switch II domains. A number of proteins have structural and mechanistic similarities as well as evolutionary relationships with the NifH protein, notable among them being: light independent protochlorophyllide (Pchlide) reductase (ChlL/FrxC or bChL), arsenite pump ATPase (ArsA), 2-hydroxyglutaryl dehydratase Component A (CompA) involved in glutamate degradation and MinD that functions in spatial regulation of cell division. Although involved in very diverse biological processes, these proteins share an underlying common structural framework. This review mainly focuses on the structural similarities of these proteins with the NifH protein and discusses recent reports of complementation studies involving NifH and few of the proteins mentioned.
Highlights
The nitrogenase enzyme is a two-component system that consists of the iron protein (Fe-protein or NifH) and molybdenum-iron protein (MoFe-protein) working in concert to effect nitrogen reduction[1,2,3,4]
For the dehydratase system, Component A (CompA) is the site of ATP hydrolysis and is the electron donor to the sec component, CompD[19], arsenite pump ATPase (ArsA) exists in a complex with the ArsB protein forming an arsenite-antimonite [As(III)/Sb(III)]- translocating ATPase
The protein sequence comparison of NifH, ArsA, ChlL, CompA and MinD gives an overview of their similar regions (Fig. 1)
Summary
The nitrogenase enzyme is a two-component system that consists of the iron protein (Fe-protein or NifH) and molybdenum-iron protein (MoFe-protein) working in concert to effect nitrogen reduction[1,2,3,4]. For the dehydratase system, CompA is the site of ATP hydrolysis and is the electron donor to the sec component, CompD[19], ArsA exists in a complex with the ArsB protein forming an arsenite-antimonite [As(III)/Sb(III)]- translocating ATPase.
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