Abstract
Beyond the nodule symbiosis process, some endophytic bacteria can convert the atmospheric nitrogen to available form for their own and the inhabiting host plants. These bacteria possess a nitrogenase enzyme complex involved in producing ammonia from atmospheric dinitrogen. The nitrogenase reductase subunit of the enzyme complex is governed by the nifH gene and is being used as a suitable marker. In the present study, the nifH gene containing endophytic bacteria had been identified from two sweet potato cultivars grown in different locations. Both the cultureindependent and culture-dependent methods were used to identify the nitrogen-fixing bacteria in sweet potatoes. The nifH sequences closest to Azospirillum, Bradyrhizobium, Herbaspirillum, Klebsiella, Kluyvera, Paenibacillus, Pseudacidovorax, Rhizobium and Sphingomonas were identified in this study. It was supposed that soils and varieties have more effects than climatic factors on nitrogen-fixing bacteria of sweet potato. These nitrogen-fixing bacteria could be utilized to develop biofertilizers helpful for sustainable agriculture.
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