Abstract
<b>Objective:</b> We aimed to investigate the role of nicotine as a cause of EBD and EMT in human bronchial epithelial cells (HBEC) and asthmatic bronchial epithelial cells (AHBEC). <b>Methods:</b> We used HBEC and AHBEC commercial cell line cultures for this study and treated with 6x10-6 mol/l nicotine. We evaluated nicotine treated (NT) and nicotine non-treated (NNT) HBEC and AHBEC at 24, 48 and 72 hours. We measured E-cadherin (E-cad), N-cadherin (N-cad), α-smooth muscle actin (α-SMA) with Western Blot and levels of thymic stromal lymphopoietin (TSLP), Wnt3a as well as transforming growth factor-β1 (TGF-β1) with ELISA in these cultures. <b>Results:</b> Comparison of NT with NNT HBEC showed lower N-cad levels at 24 hours (p=0.043); no significant difference was detected at 48 and 72 hours. N-cad was found to be higher in non-NT AHBEC versus HBEC at 24 hours (p=0.046). Moreover, comparison of NT HBEC with AHBEC showed that N-cad was higher in AHBEC at 24 hours (p=0.046). However, there was no significant difference between the groups at 48 and 72 hours (table 1). <b>Conclusion:</b> Detection of higher N-cad levels in AHBEC compared to HBEC without nicotine exposure as well as during the early stages of nicotin exposure may be a sign of tendency of asthmatic epitelial cells for EMT. This may result in worse prognosis with nicotine exposure in asthmatic.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
