Nicotine signals through muscle-type and neuronal nicotinic acetylcholine receptors in both human bronchial epithelial cells and airway fibroblasts

Diane L Carlisle,Toni M Hopkins,Michele J Silhanek,James D Luketich,Autumn Gaither-Davis,Jill M Siegfried,Neil A Christie

doi:10.1186/1465-9921-5-27

Diane L Carlisle, Toni M Hopkins + Show 5 more

Open Access

https://doi.org/10.1186/1465-9921-5-27

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Abstract

BackgroundNon-neuronal cells, including those derived from lung, are reported to express nicotinic acetylcholine receptors (nAChR). We examined nAChR subunit expression in short-term cultures of human airway cells derived from a series of never smokers, ex-smokers, and active smokers.Methods and ResultsAt the mRNA level, human bronchial epithelial (HBE) cells and airway fibroblasts expressed a range of nAChR subunits. In multiple cultures of both cell types, mRNA was detected for subunits that constitute functional muscle-type and neuronal-type pentomeric receptors. Two immortalized cell lines derived from HBE cells also expressed muscle-type and neuronal-type nAChR subunits. Airway fibroblasts expressed mRNA for three muscle-type subunits (α1, δ, and ε) significantly more often than HBE cells. Immunoblotting of HBE cell and airway fibroblast extracts confirmed that mRNA for many nAChR subunits is translated into detectable levels of protein, and evidence of glycosylation of nAChRs was observed. Some minor differences in nAChR expression were found based on smoking status in fibroblasts or HBE cells. Nicotine triggered calcium influx in the immortalized HBE cell line BEAS2B, which was blocked by α-bungarotoxin and to a lesser extent by hexamethonium. Activation of PKC and MAPK p38, but not MAPK p42/44, was observed in BEAS2B cells exposed to nicotine. In contrast, nicotine could activate p42/44 in airway fibroblasts within five minutes of exposure.ConclusionsThese results suggest that muscle-type and neuronal-type nAChRs are functional in airway fibroblasts and HBE cells, that prior tobacco exposure does not appear to be an important variable in nAChR expression, and that distinct signaling pathways are observed in response to nicotine.

Highlights

Non-neuronal cells, including those derived from lung, are reported to express nicotinic acetylcholine receptors
Neuronal and muscle-type nicotinic acetylcholine receptors (nAChR) are present on human bronchial epithelial (HBE) cells and airway fibroblasts Using RT-PCR, we repeatedly detected mRNA for nAChR subunits in short-term cultures of human airway cells from bronchial biopsies
Brain mRNA was used as a positive control for neuronal-type nAChR (Figure 1 Panel A) and muscle mRNA as a positive control for muscle-type nAChR (Figure 1 Panel B), and GAPDH is included on each panel to indicate mRNA quality

Summary

Introduction

Non-neuronal cells, including those derived from lung, are reported to express nicotinic acetylcholine receptors (nAChR). Heteropentamers have been classified as either muscle nAChR, which were first identified at the neuromuscular junction, or as neuronal type, which were discovered in the central nervous system. The adult muscle type receptor contains the α1/β1/ε/δ subunits, with the β1 subunit occurring twice to make the pentamer. The α9 and α10 subunits are unique in that they can form functional homopentamers or can combine together to form a heteropentamer without a β subunit. They are different from the other subunit combinations examined because nicotine acts as a competitive antagonist to receptors containing the α9 subunit [3]

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