Abstract
Nicotine is a potent toxic substance of cigarette smoke that has been shown to influence alterations in immunity and various metabolic processes. In this study, we evaluated the effect of nicotine toxicity on lymphocyte, a critical cell of cellular immunity using different doses at different durations. Lymphocytes were isolated by centrifugation in the density gradient from the whole blood of rats. Theywere spread over the culture media, treated with different doses of nicotine (10, 100, 100mM) for different durations (6 and 12 hours) in comparison with the control. After the experimental period, lymphocytes morphology, viability, superoxide radical concentration, malondialdehyde content, superoxide dismutase levels and DNA fragmentation were measured. The findings were analysed using one way ANOVA. The marked morphological changes in lymphocytes were observed along with decrease in presence of nicotine in a dose and duration dependent manner. There was also significant decreased in the cell viability and antioxidant enzymes, level of lipid peroxidation (LP) and DNA fragmentation was increased in nicotine treated lymphocytes compared with the control. Thus, above findings indicate that nicotine treatment at different dose and duration induces cellular damage by changing cell membrane integrity.
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