Nicotine effect toward the oocyte level of rats (Rattus novergicus)

Abstract

ObjectiveTo discover the effect of some nicotine's doses to the maturity of rat's oocyte in reproductive age. MethodsThis study was using an experimental design with 3 treatment groups (subcutaneous injection of nicotine 35, 52.5 and 70 mg/kgBW/d for 7 d) and 1 control group. After nicotine exposure treatment, the oocytes of rats were taken for the histological purpose to observe the oocyte's maturity grade in germinal vesicle (GV), Germinal Vesicle Breakdown (GVBD), Metaphase I (M I), or Metaphase II (M II). All of the oocytes were stained using Aceto orcein 1%. The data was analyzed based on One-Way Analysis of Variance (α = 0.05). ResultsThe significant result got further analyzed using LSD test. The result obtained that there were no significant differences of GV and GVBD oocytes in all groups. Otherwise, the result of One-Way ANOVA showed that there was significant differences of M−II oocytes in all groups. LSD test obtained that there was significant differences between control group and 3 treatment groups, and nicotine's treatment 52.5 mg/kg body weight (BW) with 35 mg/kg BW. This research obtained that the number of GV and GVBD oocytes were lower than the control group. ConclusionThere was no M I and M II oocytes, because nicotine treatment were less than the control group.