Abstract
The effects of single doses of azaserine or puromycin on the nicotinamide adenine dinucleotides (NADs) in rat liver have been studied. Azaserine produced a very rapid decrease in NAD + NADH 2 such that 30 min after dosing the sum was only 36 per cent of the control value. The sum NADP + NADPH 2 was not affected until considerably later in the injury process. Since NADP is synthesised via NAD, the relative rate of decrease of NADP + NADPH 2 compared to NAD + NADH 2 provides some indication of the turnover rate of NADP in the affected liver. The estimate for the “turnover time” of NADP in rat liver was 5 hr. Puromycin had no significant effect on the levels of NAD, NADH 2, NADP and NADPH 2 in rat liver although the dose used is known to inhibit protein synthesis almost completely in vivo. It is concluded that enzymes concerned in the biosynthesis of NAD and NADP do not “turn-over” so rapidly that suppression of their biosynthesis for a 1 hr period significantly affects the overall content of nicotinamide adenine dinucleotides in the liver.
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