Nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase activity in the brain of a cichlid fish, with remarkable findings in the entopeduncular nucleus: a histochemical study

Abstract

In the entire brain of the African cichlid fish, Haplochromis burtoni, nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) activity was visualized histochemically in fewer nuclei compared to other teleost fish. Intensively labeled perikarya were found in the ventral hypothalamic area, the nucleus of the medial longitudinal fascicle, the nucleus of the midbrain tegmentum, the nucleus of the lateral longitudinal fascicle, the trigeminal motor nucleus and the octavolateral area. Compared to other NADPH-d labeled nuclei in the brain, we saw an unusual localization of NADPH-d activity in the rostral, dorsal, ventral and caudal part of the entopeduncular nucleus. Additionally, some isolated perikarya of different morphological appearance were found at the levels of the preglomerular nucleus, the diffuse nucleus of the lateral torus and the lateral longitudinal fascicle. A widespread distribution of labeled fibers was identified throughout the brain. The remarkable NADPH-d activity, particularly in the entopeduncular nucleus, differs significantly from the existing data on other teleosts. Taking into account the sensory functions of the entopeduncular nucleus described in other vertebrates, the massive NADPH-d activity in this nucleus may indicate an important role of NADPH-d in the modulation of sensory functions.