Abstract
Nicotiana cavicola as a host for production of recombinant proteins by Agrobacterium-mediated transient gene expression
Highlights
The plant transient expression (TE) of transgenes is a fast, reliable and simple method that extensively used for fundamental physiological research and applied biotechnological purposes [1,2,3]
This feature is distinctive for N. benthamiana, which suggests that N. cavicola along with N. benthamiana may be used for the heterologous protein production via transient expression (TE) if the gene is located in a viral based vector system
To investigate a biological potential of N. cavicola, two mentioned species were compared for several parameters: growth in greenhouse conditions, plant biomass, easiness of infiltration procedure, possibility of Agrobacteriummediated TE of transgenes and the production of desired proteins
Summary
The plant transient expression (TE) of transgenes is a fast, reliable and simple method that extensively used for fundamental physiological research and applied biotechnological purposes [1,2,3]. TE can be used for rapid production of pharmaceutically valuable proteins, including antibodies [4,5,6,7]. Rapid production of antibodies or vaccine proteins is preferable in the treatment of advanced cancer diseases or in case of season epidemic diseases. In case of TE, the transgenes are not integrated into plant genome avoiding the position effects and providing considerably increased levels of recombinant proteins [8]. The protocols for Agrobacterium-mediated TE of transgenes in Nicotiana benthamiana plants are well developed and widely applicable.
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