Abstract
Abnormal levels of ascorbic acid in the human body can induce various diseases. We reported a nickel-doped hydroxyapatite (Ni-HAp)-based biosensor for ascorbic acid and confirmed it through various spectroscopic techniques. Ni-HAp demonstrated peroxidase-like activity that was detected through the chromogenic substrate. The analyte produces a color change from blue-green to colorless. The optimized sensor has a correlation coefficient of 0.997, a limit of detection of 0.11 µM, a limit of quantification value of 0.36 µM, and a linear range from 2 to 47 µM for ascorbic acid. The synthesized sensor was used to determine ascorbic acid in spiked physiological solutions.
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