Abstract
Summary.— Eighteen patients with nickel sensitivity and 12 control patients were examined. Lymphocytes from these patients were cultured together with nickel sulphate (antigen). Evidence of cellular hypersensitivity was obtained by the following procedures: (1) Estimation of the percentage of lymphocytes transformed to lymphoblasts; (2) the uptake of C14 thymidine in the lymphocyte cultures as evidence of DNA synthesis in the “challenged” cells; (3) the macrophage migration inhibition test, using the supernatant fluid from the cultures and guinea-pig macrophages.
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