Abstract

The study was conducted to investigate the variability of parameter in water with different stages as well as to determine the effect of high CO2 on the acid-base regulation of the eel. The field survey was measuring CO2 levels in 9 swamp eel grow-out tanks. The results showed that the partials pressure of CO2 in water was 9.5 mmHg and 28 mmHg in the middle and harvest of culture cycle (9 months), respectively. The laboratory experiment was conducted with 3 treatments including control, 14 and 30 mmHg CO2. Blood was sampled at 0, 3, 6, 24, 48, and 72-h. Blood pH decreased during the first 24-h and completely recovered after 72-h with value of 7.4±0.04 at the treatment of 14 mmHg CO2. In contrast, partial pressure of CO2 in the blood and plasma HCO3- increased significantly at the treatments of 14 and 30 mmHg CO2 during hypercapnic exposure compared to the control group (p

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