Đánh giá tác dụng chống oxy hóa và bảo vệ gan trên thực nghiệm của cây muồng lùn

Abstract

Antioxidant and hepatoprotective effects of different fractions of extract from Chamaecrista pumila (Lam.) K. Larsen were investigated by employing in vitro and in vivo models, respectively. In vitro antioxidant activity of ethanol extract and its n-hexan, ethyl acetate, chloroform, n-butanol and remained water fraction extracts obtained from C. pumila was assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and superoxide radical scavenging assay. In vitro results revealed that the ethanol extract had potential DPPH radical and superoxide radical scavenging activities with an IC50 value of 18.65 (9.91-35.08) µg/ml and 12.33 (5.24-29.02) µg/mL, respectively. Among fractions extracted from ethanol extract, the ethyl acetate fraction possessed highest antioxidant property through DPPH radical scarvenging activity with an IC50value of 9.98 (6.23-16.01) µg/mL and superoxide radical scavenging activity with an IC50 value of 8.38 (6.11-11.50) µg/mL. In vivo hepatoprotective activities of ethanolic extract and ethyl acetate fraction obtained from C. pumila were investigated in mice using a model of paracetamol-induced hepatotoxicity. Ethanol extract of C. pumila at doses of 250 mg/kg and 500 mg/kg decreased levels of serum transaminases ASAT. Ethyl acetate fraction of C. pumila at doses of 250 mg/kg and 500 mg/kg significantly improved the parameters of paracetamol-induced liver injury including ASAT, ALAT and superoxide dismutase (SOD) activities; ethyl acetat fraction of C. pumila at dose of 500 mg/kg also exhibited in vivo antioxidant properties by reducing malondialdehyde (MDA) content and increasing glutathione (GSH) content.