NGS euploid embryos have higher delivery rates than those diagnosed as euploid by aCGH/SNP

Abstract

To review outcomes of all STEET procedures based on PGT-A platform used to determine Ploidy status. Retrospective review of all STEET procedures over an 8 year period at a single center. More than 3200 STEET procedures performed over an 8 year period (2011 to 2018) at a single center were reviewed based on the PGT-A platform (NGS, aCGH or SNP) utilized. Our main outcome measures were: Implantation Rate (IR), Clinical Preg rate (FH) and Live Birth (LB) rate. Only embryos reported as euploid were included in the analysis- embryos reported as mosaic or those not yielding a result were omitted. Statistical significance was determined using contingency X2 with 1 degree of freedom. TABLE 1. Comparison of STEET outcomes depending on PGT-A PlatformTabled 1NGS1aCGH + SNP2SignificanceAverage age at Freeze36.60±4.3436.54± 4.60NSImplantation rate (sacs/embryo)70.1% (1330/1897)62.4% (858/1375)P < 0.00001Clinical Preg rate (FH/embryo)66.7% (266/1897)55.9%(768/1375)P < 0.00001SAB/ Clin Preg10.3% (87/845)12.6% (97/770)NSLive Births3 (Live born/embryo)61.7% (750/1216)53.2%(657/1235)P = 0.0000221 Only included FETs of embryos with NGS performed in the Fresh IVF cycle.2 SNP cases were included with aCGH due to low number3 Live Birth rate calculated through 2017 only (results for 2018 cycles pending) Open table in a new tab 1 Only included FETs of embryos with NGS performed in the Fresh IVF cycle. 2 SNP cases were included with aCGH due to low number 3 Live Birth rate calculated through 2017 only (results for 2018 cycles pending) STEET following PGT-A via NGS resulted in a significantly higher IR compared to aCGH /SNP combined (70.1% vs 62.4%). Similarly, ongoing Pregnancy rates and LB rates were significantly improved when NGS was utilized vs aCGH or SNP. SAB rates were not significantly different between platforms but all methods reduced SAB rates compared to age matched controls without PGS (18% ) (Ref 1). STEET results in high IR, high clinical pregnancy rates and high LB rates across all age groups. However, with advances in PGT-A platforms we can continue to improve outcomes and increase safety of ART by maximizing the potential of every ET procedure. With continuing development of PGT-A platforms and interpretation methods used to determine ploidy we can further improve outcomes and safety by transferring a single embryo with the highest implantation potential every time.