Nghiên cứu điều kiện hoạt hóa enzyme protease nội tại từ thịt đầu tôm thẻ (Litopenaeus vannamei)

Abstract

The study was conducted to assess the impact on protein hydrolysis by the activity of intracellular protease from head meat of white shrimp (Litopenaeus vannamei). The research’s content includes the influence of freezing storage time on protein hydrolysis process. In addition, activation of the pre-treated intracellular protease enzyme was optimized by using a response surface methodology (RSM) with three factors: temperature, pH and time. Hydrolysis time of protein from shrimp head’meat with the activated intracellular protease was also investigated. The results showed that the freezing storage time was maximum for 6 weeks. The optimal conditions for hydrolysis were found to be a pH of 6.95 combined with a pre-treatment at 57.5°C for 3.78 minutes. At the time, the yield of hydrolysis (DH%) increased up to 41.12%; the protein content reached 44.33 mg/100g after 6 hours of hydrolysis.