Abstract
Purpose Osteosarcoma is one of the most common primary malignant, aggressive bone neoplasms. However, the mechanisms of osteosarcoma proliferation, migration, and invasion are not well understood. To explore the possible mechanism of osteosarcoma progression, we used a public database for gene analysis to identify the possible factors that are important in osteosarcoma progression. Nuclear factor interleukin 3 (NFIL3) regulated was highly expressed in sarcoma tissues. In this study, we meant to probe the function of NFIL3 in osteosarcoma proliferation, migration, and invasion. Methods The expression of NFIL3 in osteosarcoma tissues was analysed via RT-PCR and immunohistochemistry staining. In order to elucidate the function of NFIL3 in osteosarcoma, we performed cell growth assays and colony formation assays to explore the role of NFIL3 in proliferation in osteosarcoma cells. Futhermore, we analysed osteosarcoma cell migration and invasion via wound healing assays and transwell migration and invasion assays. Results NFIL3 is overexpressed in osteosarcoma tissues; 15 of the 20 osteosarcoma tissues analysed highly expressed NFIL3. Our in vitro experiments confirmed that NFIL3 promoted the proliferation of M6-63 and SaOS2 cells (P < 0.01). In addition, NFIL3 promoted the migration and invasion of osteosarcoma cells (P < 0.05). Conclusion NFIL3 is highly expressed in osteosarcoma tissues and thus promotes the proliferation, migration, and invasion of osteosarcoma cells. NFIL3 is potential to become a new target for development of novel treatment strategies of osteosarcoma.
Highlights
Osteosarcoma is one of the most frequent malignant bone tumours, and it is most prevalent in children and young adults [1], accounting for 20% of primary bone cancers; approximately 50% of tumours are found in the proximal tibial and distal femoral areas [2]
After a series of studies, we provide the first evidence that the expression levels of Nuclear factor interleukin 3 (NFIL3) are consistently increased in osteosarcoma tissues
NFIL3 is Highly Expressed in Osteosarcoma. e expression level of NFIL3 in sarcoma tissue and normal bone tissues was compared through public database analysis, and NFIL3 was highly expressed in sarcoma tissues (Figure 1(a))
Summary
Osteosarcoma is one of the most frequent malignant bone tumours, and it is most prevalent in children and young adults [1], accounting for 20% of primary bone cancers; approximately 50% of tumours are found in the proximal tibial and distal femoral areas [2]. Osteosarcoma is highly aggressive [3, 4], with rapid growth and early metastasis. Patients with lung metastatic disease have a worse prognosis with a five-year survival rate of approximately 20% at the time of diagnosis [5–7]. Surgical treatment combined with preoperative and postoperative chemotherapy is the main treatment for osteosarcoma. Even after the combination of traditional surgical treatment and neoadjuvant chemotherapy, there are still remaining problems such as reduced patient quality of life and tumour recurrence. It is urgent to elucidate the underlying mechanisms of osteosarcoma progression
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