NF-κB activity and pathologic changes in mice with hepatic fibrosis after treated with NF-κB antisense oligonucleotide

Abstract

Objective To observe the effects of nuclear factor-κB antisense oligonucleotide (NFkB p65 ASON) on NF-κB activity and hepatic fibrosis in liver tissues of mice with hepatic fibrosis,and study the possible mechanism of NF-κB p65 ASON treating hepatic fibrosis. Methods A total of 64 BALB/c mice were divided into normal group,model group,NF-κB p65 ASON-treated group,sense oligonucleotide (SON) -treated group, missense oligonucleotide (MSON ) -treated group, control group of NF-κB p65 ASON, control group of SON and control group of MSON (n=8 each). Gel mobility shift assay (EMSA) was used to detect the DNA binding activity of NF-κB. Masson staining was used to observe the degree of hepatic fibrosis. Results The treatment of NF-κB p65 ASON could lower the NF-κB activity (12411.75 ±502.78),and fibrosis pathological scores (1. 13 ±0.64). As compared with model group (16346.88 ±449.05,2.88 ±0.64) .there was significant difference (P 0. 01). Conclusion NF-κB p65 ASON can inhibit the NF-κB activity of the mice with hepatic fibrosis efficiently,and lessen the severity of hepatic fibrosis. Key words: Hepatic fibrosis; NF-κB; Antisense oligonucleotide; Pathology