NF-α, IL6, IL1-α, IL-10 and TGF-β1 Gene Expression in Iranian Acute Myeloid Leukemia Patients

Abstract

Acute myeloid leukemia is a heterogeneous clonal disorder of blood-producing cells that are among the most common malignant disorders in adults. The purpose of this study was to examine the gene expression of TNF-α, IL6, IL1-α, IL-10, and TGF-β1 at diagnosis in acute myeloid leukemia. The current study included 50 patients with acute myeloid leukemia and 50 control subjects. ELISA was used to determine the serum concentrations of TNF-α, IL6, IL1-α, IL-10, and TGF-β1. After cDNA synthesis and RNA extraction, the expression of TNF-α, IL6, IL1-α, IL-10, and TGF-β1 genes was determined using real-time PCR (ΔΔCT computational). Statistical analysis was conducted with Statistical Package for the Social Sciences 19, and P<0.05 was deemed statistically significant. The mean serum levels of TNF-α, IL6, IL1-α, IL-10, and TGF-β1 were lower in patients with acute myeloid leukemia than in the control group. In addition, cytokine mRNA expression in peripheral blood mononuclear cells was significantly lower in AML patients compared to the control group (p<0.0001). Changes in TNF-α, IL6, IL1-α, IL-10, and TGF-β1 were associated with acute myeloid leukemia, according to the findings. The current study supports the use of cytokines as diagnostic biomarkers for acute myeloid leukemia.