Abstract

Hidden information lying underneath the genetic material is yet to be explored. Deciphering genetic information is the basic and primary step in bioscience research. For many years, capillary electrophoresis (CE)-based Sanger’s method prevailed in the scientific world for elucidation of genetic information. Due to lack in resolution, throughput, scalability, speed, and efficiency, it has now been replaced by spectacular next-generation sequencing (NGS) technologies since 5 years. Applications of NGS technologies in the field of plant biology is genome-wide scan for variants, rapid parallel sequencing, marker discovery, epigenetics, transcriptomics, de novo sequencing, resequencing, and high-resolution mapping in less time and money. This chapter briefly describes NGS technologies and utilization of these technologies in studying plant genome for its improvement and better development.

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