Abstract
Non-model plants i.e., the species which have one or all of the characters such as long life cycle, difficulty to grow in the laboratory or poor fecundity, have been schemed out of sequencing projects earlier, due to high running cost of Sanger sequencing. Consequently, the information about their genomics and key biological processes are inadequate. However, the advent of fast and cost effective next generation sequencing (NGS) platforms in the recent past has enabled the unearthing of certain characteristic gene structures unique to these species. It has also aided in gaining insight about mechanisms underlying processes of gene expression and secondary metabolism as well as facilitated development of genomic resources for diversity characterization, evolutionary analysis and marker assisted breeding even without prior availability of genomic sequence information. In this review we explore how different Next Gen Sequencing platforms, as well as recent advances in NGS based high throughput genotyping technologies are rewarding efforts on de-novo whole genome/transcriptome sequencing, development of genome wide sequence based markers resources for improvement of non-model crops that are less costly than phenotyping.
Highlights
Reviewed by: Xiyin Wang, North China University of Science and Technology, China Dongying Gao, University of Georgia, USA
Further genome sequencing of other plants was based on the idea that a single species among related plant species that share some similarities is chosen as a model, studied as a representative and information gathered can be applied to related organisms as required but Tagu et al (2014) noted that model organisms are often not archetypal and do not replicate the biology of their close relatives or even the wide diversity of living mechanisms
From the foregoing, it is clear that the cost effective and timely sequencing provided by different next generation sequencing (NGS) technology platforms has impacted positively in advancing the course of non-model plants which earlier had no place in genomics
Summary
The complex genome of Gossypium hirsutum has been elucidated in this study, which was proven difficult to sequence owing to its complex allotetraploid (AtDt) genome. The first published draft genome of an agricultural weed which is a useful genomic resource for understanding weediness and the evolution of herbicide resistance as well as development of control strategies. The study demonstrated the use of an inbred genotype derived from five generations of selfing to reduce heterozygosity and identified candidate genes which will be useful for further studies on biochemical pathways and cellular processes as well as development of molecular markers for breeding. 429.25x 437.65 Mb 98.60 301.04 kbp 33.95 kbp 32,808 The study provides insights into. The study gave an insight into the eggplant genome structure and will be a milestone for understanding unexplored species of the Solanaceae. The study which utilized two cultivars suggested the significance of historical human selection process for enhancing aroma and bitterness biosyntheses in hop cultivars, and as well serve as crucial information for breeding varieties with high quality and yield
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