Abstract
We used Illumina paired-end sequencing to isolate and characterize microsatellites of Canthon cyanellus, a Neotropical roller dung beetle, encompassing several lineages within its distribution range. We examined C. cyanellus specimens collected at eight different localities in Mexico (two or three specimens per locality). We initially performed amplification tests with 16 loci, but two of which were unsuccessful. The 14 remaining microsatellites were polymorphic, with 2-16 alleles each. The expected and observed heterozygosity ranged from 0.11 to 0.76 and from 0.20 to 0.78, respectively. These microsatellites will help to assess structure at the population and lineage levels, identify zones of potential hybridization between lineages, and draw a more precise geographic delimitation of C. cyanellus lineages.
Highlights
We examined C. cyanellus specimens collected at eight different localities in Mexico
Canthon cyanellus Le Conte, 1859 is a Neotropical carrion roller beetle in the Subfamily Scarabaeinae; its distribution range stretches from the north of Brazil to Texas [1, 2]
Canthon cyanellus cyanellus LeConte, 1859 is distributed in Mexico along the Pacific and Gulf of Mexico slopes to Texas; it is mostly green, but green- or blue-colored specimens can be found in the northern plains of the Gulf of Mexico [3]
Summary
Canthon cyanellus Le Conte, 1859 is a Neotropical carrion roller beetle in the Subfamily Scarabaeinae; its distribution range stretches from the north of Brazil to Texas [1, 2]. The cuticular color of Canthon cyanellus varies widely across its range, from predominantly reddish-brown mostly in northern South America and Central America to entirely green or even blue in tropical regions of Mexico and Texas. Canthon cyanellus sallei is distributed from Guatemala to Peru [4]; its dorsal surface is predominantly reddish-brown with greenish shades, elytra reddishbrown with greenish-black markings, and disc of the pronotum light colored, mainly reddish or yellowish-brown. We used Illumina paired-end-sequencing to isolate and characterize microsatellites of Canthon cyanellus, a Neotropical roller dung beetle encompassing several lineages within its distribution range
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