Newly discovered features of the updated sequence of royal jelly protein RJP571; longer repetitive region on C-terminus and homology toDrosophila melanogasteryellow protein

Abstract

SUMMARYThe previously published nucleotide sequence of complementary DNA (cDNA), coding for royal jelly protein RJP57-1, contained errors that were corrected by resequencing of the cDNA. The main error, the omission of a 108 bp (base pairs) long sequence on the 3' end of the cDNA was caused by misalignment of overlapping parts of the sequenced fragments in the region containing repetitive sequences. The updated sequence of RJP57-1 cDNA is 1807 bp long and encodes a protein with 544 amino acid residues including the signal peptide. The protein shows sequence homology over 385 residues with the yellow protein of Drosophila melanogaster, which is involved in the process of melanin pigmentation of Drosophila cuticle. The RJP57-1 protein sequence possesses a 100 amino acid residues long repetitive region consisting of a 20-fold repeated sequence motif XQNXX on its C-terminal part The repetitive region is interesting because of the regular distribution of cationic amino acid residues (arginine/lysine) in the la...