Abstract
Reactive electrospinning is capable of efficiently producing in situ crosslinked scaffolds resembling the natural extracellular matrix with tunable characteristics. In this study, we aimed to synthesize, characterize, and investigate the in vitro cytocompatibility of electrospun fibers of acrylated poly(1,10-decanediol-co-tricarballylate) copolymer prepared utilizing the photoreactive electrospinning process with ultraviolet radiation for crosslinking, to be used for cardiac tissue engineering applications. Chemical, thermal, and morphological characterization confirmed the successful synthesis of the polymer used for production of the electrospun fibrous scaffolds with more than 70% porosity. Mechanical testing confirmed the elastomeric nature of the fibers required to withstand cardiac contraction and relaxation. The cell viability assay showed no significant cytotoxicity of the fibers on cultured cardiomyoblasts and the cell-scaffolds interaction study showed a significant increase in cell attachment and growth on the electrospun fibers compared to the reference. This data suggests that the newly synthesized fibrous scaffold constitutes a promising candidate for cardiac tissue engineering applications.
Highlights
Myocardial Infarction (MI) affects almost one million patients yearly, with a development rate to heart failure of more than 30% of the cases in the United States
Tissue engineering (TE) using biomaterial scaffolds currently emerged as a promising treatment strategy by providing cells with a favorable environment for proper growth and proliferation in a function resembling that of the extra cellular matrix (ECM) [4]
We have previously reported on the successful synthesis, characterization, and in vivo biocompatibility testing of a new family of poly(diol-co-tricarballylate) thermoset elastomers for TE and drug delivery applications [25,26,27,28]
Summary
Myocardial Infarction (MI) affects almost one million patients yearly, with a development rate to heart failure of more than 30% of the cases in the United States. Current conventional therapies such as coronary angioplasty, cardiac transplantation, and thrombolytic drugs either require complex surgery or finding an appropriate donor, while thrombolytic therapy is not efficient in almost half of the treated population [1]. Indirect immobilization of stem cells and endomyocardial injection were examples of recent alternative regenerative medicine approaches. One more important function to be taken into consideration when developing any scaffold for the regeneration of cardiac tissue is its ability to support the environment capable of possessing the contractile properties of the cardiac tissue and the anisotropic structure of myocardial architecture [8]
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