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Abstract
Endothelin-1 and Endostar are both significant for the progression, proliferation, metastasis and invasion of cancer. In this paper, we studied the effect of ET-1 RNAi and Endostar in PC-3 prostatic cancer cells. The lentiviral vector was used in the establishment of ET-1 knockdown PC-3 cells. Progression and apoptosis were assessed by CKK-8 and flow cytometry, respectively. Transwell assay was used to estimate invasion and signaling pathways were studied by Western blotting. ET-1 mRNA and protein in ET-1 knockdown PC-3 cells were reduced to 26.4% and 22.4% compared with control group, respectively. ET-1 RNAi and Endostar both were effective for the suppression of progression and invasion of PC-3 cells. From Western blotting results, the effects of ET-1 regulation and Endostar on PC-3 cells were at least related to some signaling pathways involving PI3K/Akt/Caspase-3, Erk1/2/Bcl-2/Caspase-3 and MMPs (MMP-2 and MMP-9). Furthermore, combined treatment of ET-1RNAi and Endostar was found to be more effective than single treatment. Both ET-1 RNAi and Endostar can inhibit the progression and invasion of PC-3 cells, but combined treatment might be a better therapeutic schedule.
Highlights
Prostatic cancer, as a malignant tumor in older men, is the second most common cancer in men and the number of people killed is only after lung cancer in west Countries (Liu et al, 2013)
A) The results of transwell assay for the invasion of PC-3 cells
We might safely draw the conclusion that Endostar and ET-1 siRNA could suppress proliferation and induced apoptosis of PC-3 cells, and these two treatment were better to combination for PC-3 Cells and Modulation of Signaling Path in PC-3
Summary
As a malignant tumor in older men, is the second most common cancer in men and the number of people killed is only after lung cancer in west Countries (Liu et al, 2013). According to the results in many papers, the abundant expression of ET-1 could result in cancer cells proliferation, anti-apoptosis, angiogenesis, invasion and metastasis (Ha et al, 2011; Bagnato and Rosano, 2012; Hinsley et al, 2012; Kalles et al, 2012). Endothelin-1 and Endostar are both significant for the progression, proliferation, metastasis and invasion of cancer. We studied the effect of ET-1 RNAi and Endostar in PC-3 prostatic cancer cells. ET-1 RNAi and Endostar both were effective for the suppression of progression and invasion of PC-3 cells. From Western blotting results, the effects of ET-1 regulation and Endostar on PC-3 cells were at least related to some signaling pathways involving PI3K/Akt/Caspase-3, Erk1/2/Bcl-2/Caspase-3 and MMPs (MMP-2 and MMP-9). Conclusions: Both ET-1 RNAi and Endostar can inhibit the progression and invasion of PC-3 cells, but combined treatment might be a better therapeutic schedule
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