Abstract

Technologies that emerged during the last decade as new tools occasionally represent fundamentally new means of genome modification, which, in addition to the scientific novelty, faces legislators with new challenge by giving a new meaning to both the biochemical/molecular biological and legal meaning to genetically modified organisms (GMOs). Emerging plant genetic technologies are categorized as zinc finger nuclease technology; oligonucleotide directed mutagenesis; cisgenesis and intragenesis; RNA-dependent DNA methylation by RNA interference; grafting on genetically modified rootstock; reverse breeding; agro-infiltration; and synthetic genomics. Although all these methods apply biotechnology processes to create new plant varieties, it is debated whether all result in GMOs according to the current legal definition. Official risk assessment of these technologies is a task of outstanding weight of the authority.

Highlights

  • New applications in agricultural biotechnology not set in the EU 2001/18/EC Directive

  • oligonucleotide directed mutagenesis (ODM) is capable of creating targeted mutations of and legal meaning to genetically modified organisms a few nucleotides, using 20-100 base long (GMOs)

  • Cisgenesis and intragenesis differ from transgenesis used for the (i) zinc finger nuclease (ZFN) technology; preparation of first generation GM plants in using (ii) oligonucleotide directed mutagenesis (ODM); genes with their introns, regulating (iii) cisgenesis and intragenesis; elements or gene fragment from the same

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Summary

Introduction

New applications in agricultural biotechnology not set in the EU 2001/18/EC Directive Keywords – emerging technologies, genetically modified organisms, zinc finger nuclease technology, oligonucleotide directed mutagenesis, cisgenesis and intragenesis, RNA-dependent DNA methylation by RNA interference, grafting on GM rootstock, reverse breeding, agro-infiltration, synthetic genomics

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