Abstract
Column screening is an integral first step in purifying natural products. Efficient column screening allows facile and rapid purification of active components for further purification. The utilization of automated flash chromatography offers opportunities to streamline the column screenings of extracts. Combining pre-packed columns of a variety of chemistries with wide-polarity range chromatography is the first step in streamlining the process. Useful column chemistries include silica, ion-exchange, alumina, C18, and diol. Coincident to streamlining column screening is the incorporation of simultaneous detection techniques such as UV-Vis, mass spectrometry, and ELSD. Utilizing this enhanced automated procedure not only allows rapid purification and early correlation with previously discovered compounds, but also enables the researcher to focus on potentially novel active targets. The purification of extracts from the fruits of Capsicum annuum using silica, alumina, Diol, and reversed phase C18 columns establishes a model protocol for other extracts. Using this same example the advantages of simultaneous multiple detection techniques will be illustrated.
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