New Sustainable Process for Hesperidin Isolation and Anti-Ageing Effects of Hesperidin Nanocrystals.

Danijela Stanisic,Amanda F Costa,Roney V Dos Santos,Nelson Durán,Leticia H B Liu,Ljubica Tasic

doi:10.3390/molecules25194534

Danijela Stanisic, Amanda F Costa + Show 4 more

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https://doi.org/10.3390/molecules25194534

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Abstract

Hesperidin, a secondary orange (Citrus sinensis) metabolite, was extracted from orange bagasse. No organic solvents or additional energy consumption were used in the clean and sustainable process. Hesperidin purity was approximately 98% and had a yield of 1%. Hesperidin is a known supplement due to antioxidant, chelating, and anti-ageing properties. Herein, hesperidin application to eliminate dark eye circles, which are sensitive and thin skin regions, was studied. In addition, the proposed method for its aqueous extraction was especially important for human consumption. Further, the most effective methods for hesperidin nanonization were explored, after which the nanoemulsions were incorporated into a cream formulation that was formulated for a tropical climate. Silky cream formulations (oil in water) were tested in vitro on artificial 3D skin from cultured cells extracted from skin residues after plastic surgery. The proposed in vitro assay avoided tests of the different formulations in human volunteers and animals. It was shown that one of the nanonized hesperidin formulations was the most skin-friendly and might be used in cosmetics.

Highlights

Hesperidin (HSD) is a secondary plant metabolite and one of the principal bioflavonoids of citrus fruits
HSD is known for valuable bioactivity and can act as an antioxidant [3,4,5,6,7,8,9,10], anti-inflammatory [3,9,10], hypolipidemic [8], vasoprotective [3,4,8,11], and anticancerogenic [3,9] agent
Yield of Hesperidin Extracted from Humid Orange Peel—Green Method

Summary

Introduction

Hesperidin (HSD) is a secondary plant metabolite and one of the principal bioflavonoids of citrus fruits. This flavanone and its aglycone form, hesperetin (HST), are present in relatively high quantities (1–2%), especially in sweet immature oranges (Citrus sinensis) [1,2]. HSD (Figure 1) is proposed, after different in vitro studies, as a plant defense molecule and a potent antioxidant [7,13]. It is water soluble just at high pH, dimethyl sulfoxide, and pyridine, giving yellow and clear solutions. HSD is partially soluble in methanol and glacial acetic acid, and almost insoluble in acetone, chloroform, and benzene [12,14]

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