New surface sterilization protocols for isolation of endophytic bacteria from plants (black turtle beans, peas, and barley)

Abstract

The aim of this study was to the isolate the endophytic bacteria, optimize its isolation procedure. Ethanol, sodium hypochlorite, and mercuric chloride at various concentrations and duration were employed to optimize the surface sterilization for the isolation of endophytes from Phaseolus vulgaris, Pisum sativum, and Hordeum vulgare. A total of 21 endophytic bacteria have been isolated from three plants. Combination of 2% sodium hypochlorite, 70% ethanol, and 0.1% mercuric chloride was found effective for the surface sterilization of Phaseolus vulgaris and Pisum sativum. In case of Hordeum vulgare, 70% ethanol and 2% sodium hypochlorite was found suitable for the surface sterilization. Ethanol, sodium hypochlorite, and mercuric chloride were found effective decontaminating agents in optimum condition.