Abstract
Four new metallofluorescent indicators, analogous to calcein but prepared from glycine instead of iminodiacetic acid, have been synthesized and studied by thin-layer chromatography. In addition to fluorescein itself, three isomers of dichlorofluorescein were used in the syntheses. The product obtained with 2',7'-dichlorofluorescein, bis-4',5'-N,N-glycinemethylene-2',7'-dichlorofluorescein, is recommended as a fluorescent indicator for the titration of copper(II) with EDTA solutions.
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